• Proper editing of scientific images for publications and good scientific image handling practice
• Understanding all aspects of digital images (e.g. file formats, metadata, bit depth, histogram)
• Proper merging of fluorescent channels and use of pseudo-colors and calibration bars
• Proper application of 2D projections from 3D data
• Optional: short glance on 3D reconstruction options in Fiji/ImageJ
• Scaling images to units (e.g. µm) via metadata and adding scale bars
• Uneven lighting correction methods for visualization
• Background correction methods
• Proper contrast adjustment respecting good scientific practice limits
• Avoiding data alterations when exporting images for publication figures
• Introduction to the vector graphic software Inkscape for efficient publication figure creation
• Creating example figures